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Online Journal of Veterinary Research©
Volume 8 : 79-84, 2004.
E. coli infection and antibiotic resistance in diseased and healthy broilers
Ahmad MD, Rauf A, Rai MF. E. coli infection and antibiotic resistance in diseased and healthy broilers, Online J Vet Res, 8:79-84, 2004. The rate of Escherichia coli infection and antibiotic resistance of isolates from diseased and healthy broilers at slaughter age was evaluated. Of 103 strains of E. coli, 52 were isolated from moribund birds submitted for postmortem examination and 51 from the intestinal contents of the healthy birds brought from different slaughter points. The in vitro pathogenicity of E. coli was determined by growth characteristics of isolated strains on Congo-red medium. The pathogenic strains were identified by the production of brick red colonies on the medium after 48 hours of incubation at 37 oC. These isolates were also tested for in vitro antibiotic susceptibility test using disc diffusion technique. All the strains tested were sensitive to enorfloxacin with an inhibitory zone of 2.11±0.20 cm. Norfloxacin was the second antibiotic of choice (2.11±0.9 cm). The majority of the isolates were highly resistant to erythromycin and trimethoprim.
KEY WORDS: Escherichia coli, postmortem, slaughter age, broilers, antibiotic resistance