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Online Journal of Veterinary Research © 

 

Volume 14 (2): 188-193, 2010

Molecular evidence of Babesia spp. in cattle in the Philippines

 

Jerienil C. Foronda1, Waren N. Baticados1*(DVM, PhD) and Abigail M. Baticados1 (DVM)

 

1Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines 4031.

ABSTRACT

 

Foronda, JC, Baticados WN, Baticados AM., Molecular Evidence of Babesia spp. in Cattle in the Philippines. Online J Vet Res 14 (2): 188-193, 2010. Molecular and parasitological techniques were conducted to detect the presence of Babesia spp. in cattle in Quirino Province, Philippines. A total of 246 field bovine blood samples were collected and subjected to blood parasite examination (BPE) and polymerase chain reaction (PCR) assay. None   were found positive for Babesia spp using BPE. However, detectable ~650 bp amplicons using Babesia genus-specific primers were obtained with PCR. An overall parasite prevalence rate of 2.03% was recorded in the province of Quirino. In addition, PCR positive animals were mostly females belonging to age group ≤ 1 year old. This is the first reported molecular evidence of bovine babesiosis in the Philippines.

 

 Key words: Babesia, Polymerase chain reaction, Cattle, Philippines, Quirino Province

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