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OJVRTM
Online Journal of Veterinary Research©
Volume 9 : 6-11, 2005
Zahoor MA, Hussain I,
Mahmood MS
Molecular Virology Laboratory, Department of
Microbiology,
ABSTRACT
Zahoor MA, Hussain I,
Mahmood MS, One step RT-PCR for detection of Infectious Bursal Disease Virus, Online
J Vet Res, 9 : 6-11, 2005. A
simple method for the rapid detection of infectious bursal disease virus (IBDV)
was developed employing a single-tube reverse transcription–polymerase chain
reaction (RT-PCR). The method utilized a single buffer system for both RT and
PCR and was performed without interruption as a single thermal cycling program.
A set of primers was used to amplify 743bp fragment of the VP2 gene from the
nucleotides 701 to 1444. The method was applied to detect IBDV in 26 bursa
samples. Twenty one samples were found to contain the IBDV genome. The results
of the present study indicated that the single-tube RT-PCR may be a useful
method for detecting IBDV in bursa tissue samples from suspected cases in
epidemiological investigations.
Key Words:
IBDV; Rapid Detection; One-Step RT-PCR; Clinical Samples