©1994-2005 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.
Online Journal of Veterinary Research©
Volume 9 : 6-11, 2005
Zahoor MA, Hussain I, Mahmood MS
Molecular Virology Laboratory, Department of
Zahoor MA, Hussain I, Mahmood MS, One step RT-PCR for detection of Infectious Bursal Disease Virus, Online J Vet Res, 9 : 6-11, 2005. A simple method for the rapid detection of infectious bursal disease virus (IBDV) was developed employing a single-tube reverse transcription–polymerase chain reaction (RT-PCR). The method utilized a single buffer system for both RT and PCR and was performed without interruption as a single thermal cycling program. A set of primers was used to amplify 743bp fragment of the VP2 gene from the nucleotides 701 to 1444. The method was applied to detect IBDV in 26 bursa samples. Twenty one samples were found to contain the IBDV genome. The results of the present study indicated that the single-tube RT-PCR may be a useful method for detecting IBDV in bursa tissue samples from suspected cases in epidemiological investigations.
Key Words: IBDV; Rapid Detection; One-Step RT-PCR; Clinical Samples