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Online Journal of Bioinformatics ©
Volume 12(1):66-73, 2011
Partial sequence clone of canine mammary tumor metalloproteinase MMP11 catalytic domain
B. V. Sunil Kumar (M.V.Sc), Meena Kataria (M.Sc,Ph.D), G.V.P.P.S. Ravi Kumar*(M.V.Sc,Ph.D), K. Aswani Kumar (M.V.Sc)
and A.P. Vishwanatha Reddy
Enzymology lab., Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122 (U.P.), India.
Sunil Kumar BV, Kataria M, Kumar R, Kumar KA, Vishwanatha Reddy AP, Partial sequence clone of canine mammary tumor metalloproteinase MMP11 catalytic domain, Online J Bioinformatics, 12(1):66-73, 2011. Partial sequence of canine MMP11 catalytic domain was cloned and sequenced (Acc o.HM037636). Nucleotide and translated protein sequence was close to Equus caballus but clustered away from rodent and primate groups. Synonymous substitution (dS) was higher than non-synonymous substitution (dN) suggesting that the catalytic domain MMP11 gene was not under positive selection and that no advantageous mutations had had any significant role in its evolutionary adaptation.
(Key words: Cloning, Characterization, Matrix metalloproteinase 11, canine.