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OJVRTM
Online Journal
of Veterinary Research©
Volume 13 (1):41-52, 2009
Characterization of the
caprine (Capra hircus) SLC11A1 gene:
innate resistance to
paratuberculosis
Liandris E1,
Gazouli M2, Ikonomopoulos J1
1Agricultural University of
Athens, Faculty of Animal Science, Laboratory of Anatomy- Physiology and 2University
of Athens, School of Medicine, Laboratory of Biology, Athens, Greece
ABSTRACT
Liandris E, Gazouli M, Ikonomopoulos J,
Characterization of the caprine (Capra hircus) SLC11A1 gene:innate
resistance to paratuberculosis, Online
J Vet Res, 13 (1):41-52, 2009. Control of paratuberculosis with current means is considered a
demanding task. Recent advances in immunology have permitted the identification
of genes, amongst which SLC11A1 (solute carrier family 11 member A1), is associated
with resistance or sensitivity to specific diseases. The aim of this study was
to determine the sequence and structure of caprine
(Capra hircus) SLC11A1 gene and the association of
its polymorphisms with innate resistance to Mycobacterium avium
subsp. paratuberculosis
(MAP). One sample of blood, serum and faeces was collected from each of 50
goats over the age of two, from a herd of 400 adult animals with a long record
of paratuberculosis. This material was used for the
sequence and structure analysis of the SLC11A1 gene and the detection of MAP
DNA and antibody using Real Time PCR and ELISA. The goat SLC11A1 gene coding
sequence consisted of 2004 bases and presented a 1648-bases Open Reading Frame
encoding a 548 residue protein. The 3' untranslated
region of the targeted gene revealed 2 microsatellites
consisting of a variable number of guanine-thymine repeats, named regions A and
B. Statistical analysis of the results indicated a significant level of
association between the B8 allele of the polymorphic region B and
the presence of MAP DNA in the faeces. The results presented here suggest that
an association of specific polymorphisms of the SLC11A1 gene with innate
resistance/sensitivity of goats to paratuberculosis
is possible.
Key words: slc11a1
gene, paratuberculosis, goats
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