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Online Journal of Veterinary Research©
Volume 9 (1):1-5, 2005
Electrometric cholinesterase determination in poultry treated with dichlorvos and carbaryl
Mohammad FK , Al-Baggou B
of Physiology, Biochemistry and Pharmacology,
Mohammad FK, Al-Baggou B, Electrometric cholinesterase determination in poultry treated with dichlorvos and carbaryl, Online J Vet Res 9 (1):1-5, 2005. An electrometric method was used for measurement of cholinesterase (ChE) activity in the plasma, brain and liver of 45 days old domestic chickens of both sexes. The ChE assay mixture contained 0.2 ml of plasma or tissue homogenate, 3 ml of distilled water, 3 ml pH 8.1 phosphate-barbital buffer and 0.12 ml of 7.5% acetylthiocholine iodide as a substrate. The one-step incubation was for 30 min at 37 ˚C. Normal reference values for ChE activity (ΔpH/30 min) in the plasma, brain and liver of the chickens were 0.47, 0.25 and 0.21, respectively. Oral administration of the organophosphorus (dichlorvos, 7 mg/kg) and carbamate (carbaryl, 100 mg/kg) insecticides in chickens induced signs of toxicosis characteristic of cholinergic overstimulation, and these signs were associated with significant decreases in plasma (62 and 29%) and brain (28 and 65%) ChE activities, respectively. The results suggest that the present electrometric method for measurement of ChE activity in chickens is efficient in monitoring the enzyme inhibition caused by organophosphorus and carbamate insecticides.
KEY-WORDS: Cholinesterase, Chicken, Dichlorvos, Carbaryl, Organophosphate, Carbamate