©1996-2012
All Rights Reserved. Online Journal of Veterinary Research
. You may not store these pages in any form except for your own personal
use. All other usage or distribution is illegal under international copyright
treaties. Permission to use any of these pages in any other
way besides the before mentioned must be gained in writing from the publisher.
This article is exclusively copyrighted in its entirety to OJVR.This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors. This journal satisfies the refereeing
requirements (DEST) for the Higher Education Research Data Collection
(Australia). Linking:To link
to this page or any pages linking to this page you must link directly to this
page only here rather than put up your own page.
OJVRTM
Online
Journal of Veterinary Research©
Volume 16 (2):53-61, 2012
Coagulase gene heterogeneity in bovine mastitic
S. aureus isolates.
Zahra Esmailnezhad DVM, Masoud Haghkhah DVM PhD, Narjes Ghaffari DVM
Department
of Pathobiology, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran.
ABSTRACT
Esmailnezhad Z, Haghkhah M, Ghaffari
N., Coagulase gene heterogeneity in bovine mastitic S. aureus isolates,
Online J Vet Res, 16 (2):53-61, 2012. S. aureus coagulase gene is a significant virulence factor and its
typing is used to identify S. aureus
genotypes. The present study was conducted to characterize coagulase
gene in bovine mastitic S. aureus
isolates. Milk samples were collected from 40 mastitic
cows and S. aureus isolates were identified by
routine biochemical tests confirmed by species specific PCR to yield products
with an expected molecular size of 1.3 kb. PCR amplification of coagulase gene in the isolates yielded single bands with a
molecular size of 700 (30%) and 900 bp (70%).
Detection of single bands by coagulase PCR showed
that the enzyme was bovine of origin, and that there was no human contamination
of mammary glands or milk. Digestion
of coagulase gene PCR products with restriction endonuclease enzymes AluI
and Hin61 yielded 3 different RFLP profiles that indicates a low heterogeneity in the coagulase
gene of the bovine mastitic S. aureus isolates.
Key Words: Coagulase, heterogeneity,
mastitis, S. Aureus
FULL-TEXT(SUBSCRIBERS) FULL-TEXT (SINGLE-ARTICLE)