©1996-2012 All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Veterinary Research©
Volume 16 (2):53-61, 2012
Coagulase gene heterogeneity in bovine mastitic S. aureus isolates.
Zahra Esmailnezhad DVM, Masoud Haghkhah DVM PhD, Narjes Ghaffari DVM
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Esmailnezhad Z, Haghkhah M, Ghaffari N., Coagulase gene heterogeneity in bovine mastitic S. aureus isolates, Online J Vet Res, 16 (2):53-61, 2012. S. aureus coagulase gene is a significant virulence factor and its typing is used to identify S. aureus genotypes. The present study was conducted to characterize coagulase gene in bovine mastitic S. aureus isolates. Milk samples were collected from 40 mastitic cows and S. aureus isolates were identified by routine biochemical tests confirmed by species specific PCR to yield products with an expected molecular size of 1.3 kb. PCR amplification of coagulase gene in the isolates yielded single bands with a molecular size of 700 (30%) and 900 bp (70%). Detection of single bands by coagulase PCR showed that the enzyme was bovine of origin, and that there was no human contamination of mammary glands or milk. Digestion of coagulase gene PCR products with restriction endonuclease enzymes AluI and Hin61 yielded 3 different RFLP profiles that indicates a low heterogeneity in the coagulase gene of the bovine mastitic S. aureus isolates.
Key Words: Coagulase, heterogeneity, mastitis, S. Aureus