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Online Journal of Veterinary Research ©
Volume 15 (5): 446-454, 2011.
Molecular epidemiology of virotypes of Escherichia coli in feces of healthy household cats
Ghanbarpour Reza1 (DVM, PhD), Akhtardanesh Baharak2 (DVM, PhD), Pakseresht Akram2 (DVM)
1Microbiology, 2Small Animal Internal Medicine Departments of Faculty of Veterinary Medicine, Shahid Bahonar University, 76169-14111, Kerman, Iran
Reza G, Baharak A, Akram P., Molecular epidemiology of virotypes of Escherichia coli in feces of healthy household cats, Online J Vet Res., 15 (5): 446-454, 2011. Escherichia coli strains are classified on the basis of their virulence genotypes into virotypes. Sixty nine E. coli isolates from feces of healthy household cats were examined to determine a selection of virulence genes. Phylogenetic groups and subgroups of the isolates were determined by PCR. In 30 (43.47%) E. coli isolates virulence genes were present alone or in combination with each other. The detected virulence genes were classified in sixteen different patterns, which were considered as different virotypes. The most common virulence gene was sfa/focD-E, which was positive in 18 (26.08%) isolates. Among the E. coli isolates, 24.63% had hly, 14.49% iucD, 14.49% papE-F, 13.04% cnf1, 1.44% stx2 and 1.44% eae genes. Ten combination patterns of the virulence genes were detected in examined isolates, of which a combination of hly, sfa, pap, cnf1 genes was the most prevalent pattern. None of the isolates contained the afaIB-C, stx1, STI, LTI, cnf2 and ipaH genes. Virotypes were distributed in four main phylogroups. Sixty nine isolates belonged to A (60.87%), B1 (17.39%), B2 (7.24%) and D (14.50%) phylogroups. Twelve isolates fell in to B1 group, whereas 57 isolates (82.61%) fell into five phylogenetic subgroups: 23.19% into A0, 37.68% into A1, 7.24% into B22, 10.14% into D1 and 4.35% into D2. None of the isolates belonged to B23 subgroup. In conclusion virulence genes were found in a relatively high percentage of E. coli isolates from feces of healthy household cats. Virotypes differed phylogenetically and could be excreted into the external environment and transmitted to susceptible hosts.
Key words: Escherichia coli; virotype, genotype, cat