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©1996-2009 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors.

OJVRTM

Online Journal of Veterinary Research©

Volume 8:22-32, 2004.

Peptide AS-48 (Enterococcus faecalis) for prevention and

treatment of mastitis in dairy cows

 

Davidse EKa, Balla Ea, Holzapfel WHb, Muller CJCc,  Cloete SWPc, Dicks LMTa

 

aDepartment of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa, bInstitute of Hygiene and Toxicology, Federal Research Centre for Nutrition, Haid-und-Neu-Str. 9, 76131, Karlsruhe, Germany, cDepartment of Agriculture, Private Bag X1, 7607 Elsenburg, South Africa

ABSTRACT

 

Davidse EK,  Balla E, Holzapfel WH,  Muller CJC, Cloete SWP,  Dicks LMT Peptide AS-48 from Enterococcus faecalis for prevention and treatment of mastitis in dairy cows, Online J Vet Res 8:22-32, 2004.  Peptide AS-48, produced by Enterococcus faecalis FAIRE 92, inhibited the growth of a Staphylococcus aureus strain isolated from mastitic milk. Peptide AS-48 was isolated from the cell-free supernatant by using a combination of Triton X-114 phase partitioning and cation exchange chromatography. The partially purified peptide was liposome-encapsulated at a yield of 400 AU (arbitrary units)/ml and injected into infection-free quarters of healthy Holstein cows. These quarters were then infected by injecting 2 ml of the S. aureus pathogen (3.3 x 103 cfu/ml) through the teat canals. Control udders not pre-treated with AS-48 were also injected with the same level of S. aureus. From the second day after milking to day 7, the somatic cell count (SCC) in milk from udders that have been pre-treated with liposome-encapsulated peptide AS-48 decreased by 60%. The viable cell numbers of S. aureus in milk from pre-treated udders remained more-or-less the same over the 7-day period (1 x 102 cfu/ml), whereas the S. aureus cell numbers in milk from untreated udders increased to 8 x 102 cfu/ml. When S. aureus-infected udders with a SCC higher than 5 x 105/ml were injected together with liposome-encapsulated peptide AS-48 (6 400 AU/ml), the SCC in milk from these animals decreased by ca. 85% and the number of viable S. aureus by ca. 99%. Streptococcus agalactiae and Streptococcus dysgalactiae, isolated from mastitic milk, were also inhibited in in vitro tests, but not Escherichia coli.

 

KEYWORDS: Mastitis, Treatment, Peptide AS-48

©1996-2009 All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors.

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