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OJVRTM
Online Journal of Veterinary Research©
Volume 13
(1):1-12, 2009
Prevention of
fluoride-induced testicular disorders in male Sprague-Dawley
rats by co-administration of Vitamin E and/or ginger oil.
Elbohi KM*, Abd El-Rahman SS**.
* Department(s)
of Forensic Medicine &Toxicology and** Pathology, Faculty of Veterinary
Medicine,
ABSTRACT
Elbohi KM, Abd
El-Rahman SS., Prevention of fluoride-induced
testicular disorders in male Sprague-Dawley rats by
co-administration of Vitamin E and/or ginger oil. Online J Vet Res 13(1):1-12,
2009. This study
was conducted to investigate the influence of vitamin E and ginger oil on the
adverse effects of sodium fluoride (NaF) on male rat fertility
parameters. Five groups of male Sprague-Dawley rats
were used. Rats of Group 1 (G1) were kept as control and the other four groups
were treated with 20mgNaF/kg/day for 28 days by oral gavage.
Rats of group 2 (G2) was kept on NaF treatment only.
Another three groups were treated with Vitamin E co-administration at a dose of
20mg/100g/day, ginger oil co-administration at a dose of 20 ml/kg/d and both
Vitamin E and ginger oil co-administration at the same doses, respectively.
Results demonstrated fluoride-induced histological alterations in male genital
organs including inhibition of spermatogenesis and spermiation,
necrosis of spermatogonial cells, abnormal spermatid forms and spermatid
giant cells. Sperm count as well as sperm motility declined significantly
(p<0.001). A significant reduction (P< 0.001) in sperm viability and a
high percentage of abnormal spermatogoa were also
observed. Moreover, lowered serum and testicular testosterone levels occurred.
Co-administration of Vitamin-E and ginger oil with fluoride resulted in a
significant protection from induced testicular disorders. It was concluded that
fluoride exposure led to inhibition of testicular gametogenesis
and steroidogenesis which were protected
significantly by dietary agents like Vitamin-E and ginger oil.
Key
Words: fluoride toxicity, male fertility, ginger, vitamin E.
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