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Online Journal of Veterinary Research©
Volume 13 (1):1-12, 2009
Prevention of fluoride-induced testicular disorders in male Sprague-Dawley rats by co-administration of Vitamin E and/or ginger oil.
Elbohi KM*, Abd El-Rahman SS**.
of Forensic Medicine &Toxicology and** Pathology, Faculty of Veterinary
Elbohi KM, Abd El-Rahman SS., Prevention of fluoride-induced testicular disorders in male Sprague-Dawley rats by co-administration of Vitamin E and/or ginger oil. Online J Vet Res 13(1):1-12, 2009. This study was conducted to investigate the influence of vitamin E and ginger oil on the adverse effects of sodium fluoride (NaF) on male rat fertility parameters. Five groups of male Sprague-Dawley rats were used. Rats of Group 1 (G1) were kept as control and the other four groups were treated with 20mgNaF/kg/day for 28 days by oral gavage. Rats of group 2 (G2) was kept on NaF treatment only. Another three groups were treated with Vitamin E co-administration at a dose of 20mg/100g/day, ginger oil co-administration at a dose of 20 ml/kg/d and both Vitamin E and ginger oil co-administration at the same doses, respectively. Results demonstrated fluoride-induced histological alterations in male genital organs including inhibition of spermatogenesis and spermiation, necrosis of spermatogonial cells, abnormal spermatid forms and spermatid giant cells. Sperm count as well as sperm motility declined significantly (p<0.001). A significant reduction (P< 0.001) in sperm viability and a high percentage of abnormal spermatogoa were also observed. Moreover, lowered serum and testicular testosterone levels occurred. Co-administration of Vitamin-E and ginger oil with fluoride resulted in a significant protection from induced testicular disorders. It was concluded that fluoride exposure led to inhibition of testicular gametogenesis and steroidogenesis which were protected significantly by dietary agents like Vitamin-E and ginger oil.
Key Words: fluoride toxicity, male fertility, ginger, vitamin E.