si©1996-2011
All Rights Reserved. Online Journal of Veterinary Research . You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties.
Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from
the publisher. This article is exclusively copyrighted in its entirety to OJVR.This article may be copied once but may not be,
reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the
refereeing requirements (DEST) for the Higher Education Research Data
Collection (Australia). Linking:To link to this page or any pages
linking to this page you must link directly to this page only here rather than
put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Detection of foot and mouth disease by sandwich ELISA, viral isolation and multiplex PCR
Neeta Longjam , A.K.
Sarmah, Rajib Deb*1,
Basavraj Mathapati *2
V.K.Saxena1 and Tilling Tayo*3
Department of
Veterinary Microbiology, College of Veterinary Science , Assam Agricultural
University, Khanapara, Guwahati-781022 *1 Division
of Animal Biotechnology, Indian Veterinary Research Institute (IVRI), Izatnagar, UP- 243122, *2 Division of Veterinary
Virology , Indian Veterinary Research Institute (IVRI), Izatnagar,
UP- 243122, *3 Division of Animal Nutrition , Indian Veterinary
Research Institute (IVRI) , Izatnagar, UP- 243122
ABSTRACT
Longjam N, Sarmah AK,
Deb R, Mathapati B, Saxena
VK, Tayo ., Detection of foot and mouth disease
by sandwich ELISA, viral isolation and multiplex PCR, Online J Vet Res, 15 (1):
76-92, 2011. Foot-and-mouth
disease virus serotype ‘O’ in clinical samples isolated from Indian origin viz.
tongue/feet epithelium (ES) and oral swabs were detected by sandwich ELISA,
virus isolation in cell culture and multiplex PCR. Evaluation of comparative
efficiency of the tests used in the present study revealed that multiplex PCR
could detect FMD virus in the highest number of samples (65.47%) followed by
sandwich ELISA (53.57%) and virus isolation (42.85%).
Key Words: Foot-and-Mouth disease
virus serotype ‘O’; North Eastern region of India; Sandwich ELISA
;Virus isolation; Multiplex PCR