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Online Journal of Veterinary Research©
Volume 15 (1): 76-92, 2011
Detection of foot and mouth disease by sandwich ELISA, viral isolation and multiplex PCR
Neeta Longjam , A.K. Sarmah, Rajib Deb*1, Basavraj Mathapati *2 V.K.Saxena1 and Tilling Tayo*3
Department of Veterinary Microbiology, College of Veterinary Science , Assam Agricultural University, Khanapara, Guwahati-781022 *1 Division of Animal Biotechnology, Indian Veterinary Research Institute (IVRI), Izatnagar, UP- 243122, *2 Division of Veterinary Virology , Indian Veterinary Research Institute (IVRI), Izatnagar, UP- 243122, *3 Division of Animal Nutrition , Indian Veterinary Research Institute (IVRI) , Izatnagar, UP- 243122
Longjam N, Sarmah AK, Deb R, Mathapati B, Saxena VK, Tayo ., Detection of foot and mouth disease by sandwich ELISA, viral isolation and multiplex PCR, Online J Vet Res, 15 (1): 76-92, 2011. Foot-and-mouth disease virus serotype ‘O’ in clinical samples isolated from Indian origin viz. tongue/feet epithelium (ES) and oral swabs were detected by sandwich ELISA, virus isolation in cell culture and multiplex PCR. Evaluation of comparative efficiency of the tests used in the present study revealed that multiplex PCR could detect FMD virus in the highest number of samples (65.47%) followed by sandwich ELISA (53.57%) and virus isolation (42.85%).
Key Words: Foot-and-Mouth disease virus serotype ‘O’; North Eastern region of India; Sandwich ELISA ;Virus isolation; Multiplex PCR