MAIN


1996-2012 All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJBTM

Online Journal of Bioinformatics

Volume 13(2):184-191, 2012


Amino acid sequence analysis of glutamate dehydrogenase from different source organisms

 

Vivek Dhar Dwivedi1 (M.Sc.) Sarad Kumar Mishra2 (PhD).

 

 

¹Department of Bioinformatics, UCST, Dehradun ²Department of Biotechnology, DDU University, Gorakhpur. India

 

Abstract

 

Dwivedi VD, Mishra SK., Amino acid sequence analysis of glutamate dehydrogenase from different source organisms, Online J Bioinform., 13(2):184-191, 2012. Glutamate dehydrogenase (GLDH) is present in most microbes and mitochondria of eukaryotes to convert glutamate to α-ketoglutarate and vice versa. Thirty full-length amino acid sequences of GLDH from fungi, plants and animals were subjected to multiple sequence alignment (MSA), pattern and domain identification for individual amino acid composition and phylogenetic tree construction. MSA revealed 6 identical glycine, 2 glutamic acid and asparagine, and 1 aspartic acid, tyrosine, alanine, valine, serine and leucine residues in all species studied. Five identical glycine, 2 glutamic acid and proline, and 1 arginine and alanine residues were found in fungal and plants sources. Five identical glycine, 3 glutamic acid, 2 praline, 1 threonine, arginine, alanine, asparagine, 1 tryptophan and leucine residues occurred in plant and fungi. Five amino acid residue patterns of different length occurred in plant and animal GLDH. Two major sequence clusters were used to construct plant and animal and fungal philogenetic trees. The average frequency of amino acid glycine was found to be 10.2% which was high compared with other amino acids. Ten motifs unique for their group were identified.

 

Keywords: GLDH. MSA. Phylogenetic analysis. Conserved regions. Motifs. Domains. Amino acid composition.


MAIN

 

FULL-TEXT(SUBSCRIPTION)