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Online Journal of Veterinary Research©
Volume 4:99-105 1999
Immunogenicity of influenza glycoproteins
with different forms of supramolecular organization
A P Bogoyavlenskiy, V E Berezin,
V P Tolmacheva, S
Laboratory of Antivirus Protection, Institute of Microbiology & Virology, Almaty, Kazakhstan, Address for correspondence A.P. Bogoyavlenskiy: Laboratory of Antivirus Protection, Institute of Microbiology & Virology, Almaty, Kazakhstan.
Bogoyavlenskiy AP, Berezin VE, Tolmacheva VP, Khudyakova SS, Ogneva AV., Immunogenicity of influenza glycoproteins with different forms of supramolecular organization in hens, Online J Vet Res, 4:99-105, 1999. The immunogenicity of an influenza A subunit vaccine preparation administered to hens in glycoprotein micelles, complex of glycoproteins with glycoside Quil A, and glycoproteins mixed with complete Freund's adjuvant were assessed in comparative studies with formalinized whole virus. The results showed that immunization of the influenza antigen preparations induced variable levels of antibodies at 14, 21, 35, 77, 98 and 300 days postimmunization. Immune Stimulating complexes (ISCOMs) were the most efficient antigen formulation of purified FPV glycoproteins stimulating a higher level of humoral immunity than whole virus or glycoprotein micelles. ISCOMs also stimulated a high level of antibodies in egg-yolk. Egg-yolk antibody titers were correlated with the level of serum antibodies.
KEYWORDS: humoral immunity, influenza vaccines, ISCOMs