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OJVRTM®
Online Journal of Veterinary Research©
Volume 4:99-105 1999
Immunogenicity of influenza glycoproteins
with different forms of supramolecular organization
in hens
A P Bogoyavlenskiy, V E Berezin,
V P Tolmacheva, S
Laboratory of Antivirus
Protection, Institute of Microbiology & Virology, Almaty,
Kazakhstan, Address for correspondence A.P. Bogoyavlenskiy:
Laboratory of Antivirus Protection, Institute of Microbiology & Virology, Almaty, Kazakhstan.
ABSTRACT
Bogoyavlenskiy AP, Berezin
VE, Tolmacheva VP, Khudyakova
SS, Ogneva AV., Immunogenicity
of influenza glycoproteins with different forms of supramolecular organization in hens, Online J Vet Res, 4:99-105, 1999. The
immunogenicity of an influenza
A subunit vaccine preparation administered to hens in glycoprotein micelles,
complex of glycoproteins with glycoside Quil A, and glycoproteins mixed
with complete Freund's adjuvant were assessed in comparative studies with formalinized whole virus. The results showed that
immunization of the influenza antigen preparations induced variable levels of
antibodies at 14, 21, 35, 77, 98 and 300 days postimmunization.
Immune Stimulating complexes (ISCOMs) were the most
efficient antigen formulation of purified FPV glycoproteins
stimulating a higher level of humoral immunity than
whole virus or glycoprotein micelles. ISCOMs also
stimulated a high level of antibodies in egg-yolk. Egg-yolk antibody titers
were correlated with the level of serum antibodies.
KEYWORDS: humoral
immunity, influenza vaccines, ISCOMs