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OJVRTM
Online
Journal of Veterinary Research ©
Herd
immunity
in buffaloes after intranasal live gdhA
derivative P. multocida
B:2 vaccine
Rafidah O1,2, Zamri-Saad
M1, Nasip E2,
Saharee AA1
1Faculty
of Veterinary Medicine, Universiti
Putra Malaysia, and 2Department of Veterinary Services and
Animal
Industry, Kota Kinabalu, Sabah, Malaysia
Abstract
Rafidah O, Zamri-Saad
M, Nasip E, Saharee
AA., Herd
immunity in buffaloes after intranasal live gdhA
derivative P. multocida B:2, Online J Vet
Res., 15
(3): 283-290,
2011. The results of a
live attenuated gdhA derivative
P. multocida B:2
vaccine on
immunity in buffalo herds is described. Three
groups of 30 to 50 buffaloes selected from a haemorrhagic
septicaemia-endemic
and non-endemic area were used. Thirty percent of animals in each
group were given intranasal 5 ml inoculum
106 cfu/mL live attenuated gdhA
derivative P. multocida
B:2. After exposure,
vaccinated buffaloes were mingled with non-vaccinated
animals of the same group. A booster dose was given 10 months after the
first
exposure. Serum samples were randomly collected from 30% of each group
before
vaccination and at 2-month intervals for 22 months to determine herd
immune
status by ELISA. Endemic or non-endemic herd status was compared with
15
non-vaccinated controls. Results revealed high herd antibody levels in
endemic
and non-endemic areas before vaccination. After vaccination, herd
antibody
levels in both areas gradually increased to peak values by the 6th
month
and then started to gradually decline until month 10. Following booster
dose at
10th month, antibodies declined to levels similar to those
in unvaccinated
animal at 12 to 14 months. Antibody levels then recovered at
22-months (end of study). It was concluded that field vaccination using
gdhA derivative
P. multocida B:2
increased herd immunity for 8 to 10 months
before a booster dose was required.
Key words: gdhA derivative
Pasteurella multocida B:2;
immune response; field buffalo.
FULL-TEXT(SUBSCRIPTION)