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Online Journal of Veterinary Research©
Volume 8:16-21, 2004.
Combined use of g-interferon and interleukin-2 receptor assays for diagnosis of bovine tuberculosis
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL
a Department of Immunoprophylaxis and Animal Welfare, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, via Bianchi 9, 25124 Brescia, Italy b Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, via Bologna 148, 10154 Turin, Italy Corresponding Author: Dr. Massimo Amadori, Department of Immunoprophylaxis and Animal Welfare, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, via A. Bianchi, 9 25124
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL. Combined use of g-interferon and interleukin-2 receptor assays for diagnosis of bovine tuberculosis. Online Journal of Veterinary Research 8 : 16-21, 2004. The g-interferon assay for bovine tuberculosis has reached wide acceptance in many countries. Yet, less than optimal specificity in some areas and a certain percentage of inconclusive results may affect this diagnostic technique. The problem of inconclusive results can be approached by a two-stage procedure, whereby peripheral blood mononuclear cells of g-interferon dubious samples are later analyzed for expression of the interleukin-2 receptor; this had been validated as a further alternative marker of cell-mediated immunity in M. bovis-infected cattle. This way, the two assays are used sequentially on the same blood samples. As a result, further diagnostic information can be obtained without new blood samplings in the field. The results of the interleukin-2 receptor assay showed a high correlation with those of the g-interferon assay. Instead, there was no clear evidence of increased specificity under the conditions reported in this study.
KEYWORDS: Cattle, Tuberculosis, Diagnosis, g-interferon, interleukin-2 receptor