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Online Journal of Veterinary Research

 Volume 13 (2):128-135, 2009

Respiratory macrophage activity and pulmonary morphology following

exposure to benzo(a)pyrene in broilers


Latif IK1 (DVM, MS), Karim AJ1 (DVM, MS), Zuki ABZ1 (DVM, PhD), Zamri-Saad M1 (DVM, PhD, Niu JP2 (MD, MS), Noordin MM1 (DVM, MS, PhD)


1Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia,2School of Public Health, Lanzhou University, 73000 Lanzhou, China



Latif IK, Karim AJ, Zuki ABZ, Zamri-Saad M, Niu JP, Noordin MM, Respiratory macrophage activity and pulmonary morphology following exposure to benzo(a)pyrene in broilers, Online J Vet Res, 12 (2):128-135, 2009. The effect of benzo(a)pyrene (BaP) on the macrophage defense system and lung morphology in broilers was evaluated. One day age chicks were divided into 4 groups of 40. Control chicks were given tricaprylin alone intratracheally (IT), while treated groups were given BaP (viz, at 1.5μg, 150μg or 15mg/kg bodyweight) for 5 consecutive days IT. Ten birds per group were sacrificed at days 7, 14, 21 and 35. Respiratory lavage was performed immediately to procure avian respiratory macrophages (ARM). A significant decrease in the mean values of the given lavage volume, phagocytosis and intracellular killing activities along with an increase in ARM counts was observed especially in the group receiving 15mg BaP, suggesting a possible immunopulmonary toxic effect of inhaled BaP.


Keywords: benzo(a)pyrene, intra-tracheal, macrophage, toxicity, broiler