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© Online Journal of Veterinary Research
Volume 15(6): 468-475, 2011.
Multitesting of brucellosis in small ruminants
Maninder Singh MVSc, DK Singh PhD, Rupa Boral MVSc, Gita Kumari MVSc, Shriya Rawat MVSc, Ripan Biswas MVSc.
Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly (Uttar Pradesh), India
Singh M, Singh DK, Boral R, Kumari G, Rawat S, Biswas R., Multitesting of brucellosis in small ruminants, Online J Vet Res, 15(6): 468-475, 2011. The seroprevalence of brucellosis in various regions in India was determined in 398 goats and 604 sheep using the rose bengal plate (RBPT), standard tube agglutination (STAT), complement fixation (CFT) and indirect-ELISA tests. A high number of positives were found in Karnataka, Punjab and Himachal Pradesh. ELISA detected 15.77% positives in 158 , CFT 11.88% in 119, STAT 11.08% in 111 and RBPT 7.58% in 76 animals. I-ELISA detected 15.33% in 61 goats and 16.06% in 97 sheep whereas RBPT only detected 5.28% in 21 goats and 9.11% in 55 sheep. There were significant differences between sensitivities of RBPT, STAT and I-ELISA. Interestingly, the RBPT test was least sensitive in goats (62.50 %) and sheep (67.74 %) whereas I-ELISA was more sensitive at 93.55% in goats and 87.50% in sheep. I-ELISA produced the highest performance index value in both species. In goats, the highest agreement was between RBPT and CFT while in sheep it was between I-ELISA and CFT.
Keywords: brucellosis, sheep, goats, serological tests
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