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OJVRTM
© Online Journal of Veterinary Research
Volume 15(6): 468-475, 2011.
Multitesting of brucellosis in small ruminants
Maninder
Singh MVSc, DK Singh PhD, Rupa Boral MVSc, Gita Kumari MVSc, Shriya Rawat MVSc,
Ripan Biswas MVSc.
Division of
Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly (Uttar Pradesh), India
ABSTRACT
Singh M, Singh DK, Boral R, Kumari G, Rawat S, Biswas R.,
Multitesting of brucellosis in small
ruminants, Online J Vet Res, 15(6): 468-475,
2011. The seroprevalence
of brucellosis in various regions in India was determined in 398 goats and 604
sheep using the rose bengal
plate (RBPT), standard tube agglutination (STAT), complement fixation (CFT) and
indirect-ELISA tests. A high number of positives were found in Karnataka,
Punjab and Himachal Pradesh. ELISA detected 15.77% positives in 158 , CFT 11.88% in 119, STAT 11.08% in 111 and RBPT 7.58%
in 76 animals. I-ELISA detected 15.33% in 61 goats and 16.06% in 97 sheep whereas
RBPT only detected 5.28% in 21 goats and 9.11% in 55 sheep. There were
significant differences between sensitivities of RBPT, STAT and I-ELISA.
Interestingly, the RBPT test was least sensitive in goats (62.50 %) and sheep
(67.74 %) whereas I-ELISA was more sensitive at 93.55% in goats and 87.50% in
sheep. I-ELISA produced the highest performance index value in both species. In
goats, the highest agreement was between RBPT and CFT while in sheep it was
between I-ELISA and CFT.
Keywords:
brucellosis,
sheep, goats, serological tests
RAW-DATA FILE: Provided