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Online Journal of Veterinary Research


Volume 13 (2):65-72, 2009

Effect of intranasal attenuated Pasteurella multocida B:2 on haemorrhagic septicaemia in calves


Khin MN1, Zamri-Saad M1, Noordin MM1, Effendy AWM2


1Faculty of Veterinary Medicine, Universiti Putra, 2Faculty of Science and Technology, Universiti Malaysia Terengganu, Malaysia.



Khin MN, Zamri-Saad M, Noordin MM, Effendy AWM., Effect of intranasal attenuated Pasteurella multocida B:2 on haemorrhagic septicaemia in calves. Online J Vet Res., 13 (2):65-72, 2009. This report describes the effect of vaccination with attenuated P. multocida B:2 on wild-type P. multocida B:2 infection in calves. Calves were given 5 ml intranasal 107 CFU/mL live attenuated gdhA derivative of P. multocida B:2. Untreated calves were then mingled with the vaccinated Group. Control untreated calves were kept separate. After 6 weeks, all calves were challenged intra-tracheally with 107 CFU/mL of live wild-type P. multocida B:2. At Post-Mortem none of the vaccinated or mingled calves had lesions whereas controls revealed pulmonary petechial haemorrhages with acute pneumonia patches with neutrophils. Both exposed and commingled calves showed significantly (p<0.05) higher levels of serum IgG. P. multocida B:2 were successfully isolated only from the control calves of Group 3. These findings suggest that intranasal exposures to live attenuated P. multocida B:2 prevented infection by wild-type P. multocida B:2 and may have protected susceptible calves.


Keywords: Intranasal, attenuated Pasteurella multocida B:2, infection