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OJVRTM
Online Journal of Veterinary Research©
Volume 10 (2) : 134 - 145, 2006
Probiotic potential
of Avian Intestinal Lactic Acid Bacteria
SUMMARY
Mojgani N, Torshizi M, Molae M, Probiotic potential of
Avian Intestinal Lactic Acid Bacteria Online J Vet Res
10 (2) : 134-145, 2006. A total of 250 chicken intestinal specimens were screened
for the presence of Lactic acid bacteria (LAB) by routine cultural,
morphological and biochemical reactions. Based on the results, 75 isolates were
identified as LAB and were characterized for their probiotic
potential. During preliminary investigations, the viability of the isolated LAB
strains under different environmental and stress conditions was evaluated.
Three of the strains which appeared resistant to acidic pH values and high bile
salt concentrations were selected for further detailed studies. Based on the
biochemical reactions these strains were identified as, Lactobacillus acidophilus RNL26, Lactobacillus fermentum RNL 44, and Lactobacillus salivarrius RNL 49. All three strains demonstrated enhanced inhibitory
activity against gram positive species including Clostridium
perfringens, Staphylococcus
aureus and Listeria monocytogenes. Only L.fermentum RNL 44 was inhibitory towards gram negative bacteria; Salmonella
enteritidis and
S.typhimurium. The antibacterial effect exerted by L. acidophilus RNL26 and L.salivarrius
RNL 49 appeared to be due to bacteriocin
or bacteriocin like substance, as the neutralized and
catalase treated supernatant fluid of both the
strains retained their antibacterial activity. All three strains were
further characterized for their cholesterol removal, aggregation,
co-aggregation, and cell surface hydrophobicity under
in vitro conditions.
Key Words: Lactic Acid Bacteria (LAB), Invitro,
Antimicrobial, Poultry, Probiotic potential.