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Online Journal of Veterinary Research©
Volume 5 (1): 180-199, 2001.
Mitogenic reactivity of chicken intestinal intraepithelial lymphocytes to concanavalin A and its potentiation by muramyl dipeptide and immune lymphokynes during salmonella enteriditis infection.
TR Nagaraja MVSc1, TK Raghu MVSc2, P Anand Kumar MVSc PhD3, SC Mishra MVSc PhD4
1. Project Directorate on
Animal Disease Monitoring and Surveillance, Hebbal,
Nagaraja TR, Raghu TK, Anand Kumar P, Mishra SC. Mitogenic reactivity of chicken intestinal intraepithelial lymphocytes to concanavalin A and its potentiation by muramyl dipeptide and immune lymphokynes during salmonella enteriditis infection. Online J Vet Res 5: 180-199, 2001. Chicken intestinal intraepitheleial lymphocytes (iIELs) were evaluated for their mitogenic reactivity to concanavalin A (Con A) during early phase of Salmonella Enteritidis (SE) infection of the host by employing MTT colorimetric blastogenesis assay. The proliferative response of iIELs from SE-infected chicks did not alter at 24 hr and 7 days post infection (PI) compared to uninfected age-matched chicks. The effects of muramyl dipeptide (MDP) and SE-immune lympokines (SE-ILK) on iIELs in uninfected and SE-infected chicks were assessed. Administration of MDP resulted in significant increases (P < 0.01) in mitogenic reactivity of iIELs in uninfected and SE infected chicks at 8 days post treatment (PT). iIELs from uninfected and SE-infected chicks, both treated with SE-ILK (24 hr prior to infection in the latter group) showed enhanced (P < 0.01) proliferative response at 48 hr and 8 days PT.
Key words : Intestinal intraepithelial lymphocytes ; Mitogenic reactivity; lymphokines ; Muramyl dipeptide ; Salmonella Enteritidis ; chicken